Regulation of Metabolic Processes by Hydrogen Peroxide Generated by NADPH Oxidases

Hydrogen Peroxide from NADPH Oxidase Microglia Proliferation Is Regulated by

Negative regulation of NADPH oxidase-4 by hydrogen peroxide clone-5

Hydrogen peroxide-inducible clone-5 (Hic5) is a focal adhesion adaptor protein induced by the pro-fibrotic cytokine, transforming growth factor-β1 (TGF-β1). We have previously demonstrated that TGF-β1 induces myofibroblast differentiation and lung fibrosis by activation of the reactive oxygen species (ROS)-generating enzyme, NADPH oxidase 4 (Nox4). Here, we investigated a potential role for Hic...

Activation of antibacterial autophagy by NADPH oxidases.

Autophagy plays an important role in immunity to microbial pathogens. The autophagy system can target bacteria in phagosomes, promoting phagosome maturation and preventing pathogen escape into the cytosol. Recently, Toll-like receptor (TLR) signaling from phagosomes was found to initiate their targeting by the autophagy system, but the mechanism by which TLR signaling activates autophagy is unc...

Hydrogen peroxide produced by NADPH oxidases increases proline accumulation during salt or mannitol stress in Arabidopsis thaliana.

Many plants accumulate proline, a compatible osmolyte, in response to various environmental stresses such as water deficit and salinity. In some stress responses, plants generate hydrogen peroxide (H2 O2 ) that mediates numerous physiological and biochemical processes. The aim was to study the relationship between stress-induced proline accumulation and H2 O2 production. Using pharmacological a...

High-throughput screening of inhibitors of NADPH oxidases 1 High-throughput assays for superoxide and hydrogen peroxide: Design of a screening workflow to identify inhibitors of NADPH oxidases*

Recent progress characterizing the reaction mechanism(s) of fluorescent probes with reactive oxygen species has made it possible to rigorously analyze these reactive species in biological systems. We have developed rapid high throughput-compatible assays for monitoring cellular production of superoxide radical anion and hydrogen peroxide using hydropropidine and coumarin boronic acid probes, re...